| Teriparatide

Teriparatide, sold under the brand name Forteo, is a form of parathyroid hormone (PTH) consisting of the first (N-terminus) 34 amino acids, which is the portion of the hormone activating the Parathyroid hormone 1 receptor.It is an effective anabolic (promoting bone formation) agent used in the treatment of some forms of osteoporosis.Teriparatide is a recombinant human parathyroid hormone analog (PTH 1-34).It has an identical sequence to the 34 N-terminal amino acids of the 84-amino acid human parathyroid hormone.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	120	60	30	15	7.8	3.75	1.87	0
OD	0.16	0.31	0.53	0.81	1.25	1.5	1.75	2.16

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Teriparatide spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-111	99
Plasma(n=8)	82-107	100
Cell Culture Supernates(n=8)	82-114	99

 

| Linearity

Samples were spiked with high concentrations of Teriparatide and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	100-114	86-104	99-108
	Average (%)	105	97	104
1 : 4	Range (%)	82-105	96-114	84-112
	Average (%)	98	103	103